The Features of Immunoglobulin Immobilization Processes on the Surface of Magnetite/Hydroxyapatite Magnetosensitive Nanocomposite

Authors

  • A. L. Petranovska Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine
  • V. N. Mishchenko Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine
  • M. P. Turelyk Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine
  • G. M. Gun’a Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine
  • P. P. Gorbyk Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine

Abstract

Techniques of normal human immunoglobulin (Ig) immobilization on the surface of magnetite/hydroxyapatite and magnetite/hydroxyapatite/silver nanocomposites were developed. The isotherms of Ig adsorption on the surface of nanocomposites obtained in two buffer solutions, phosphate and saline, were analyzed. It was found that silver nanoparticles act as additional adsorption sites of immunoglobulin. It was shown that Ig adsorption on the surface of the nanocomposite with silver inclusions is higher than that on the surface of magnetite/hydroxyapatite in both buffer systems studied. A dependence of the kinetics of immunoglobulin desorption to a model environment on the adsorption buffer was studied.

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(1)
Petranovska, A. L.; Mishchenko, V. N.; Turelyk, M. P.; Gun’a, G. M.; Gorbyk, P. P. The Features of Immunoglobulin Immobilization Processes on the Surface of Magnetite Hydroxyapatite Magnetosensitive Nanocomposite. Him. Fiz. Tehnol. Poverhni 2010, 1, 182-186.

Issue

Vol. 1 No. 2 (2010): Chemistry, Physics and Technology of Surface / Himia, Fizika ta Tehnologia Poverhni

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