Impact of nanocomposites with prolonged release of bioactive substances on the vital activity of yeast cells
Abstract
As a result of a study on the kinetics of carbon dioxide formation during alcohol fermentation, the stimulating effect of zinc containing nanomaterials on Saccharomyces cerevisiae yeast has been found. By the method of in vivo staining with trypan blue it has been shown that film of polyurethane the least damages yeast cells among the polymer matrices without filler (polyurethane, interpenetrating nets with polyurethane and poly (2-hydroxyethylmethacrylate)). Vice versa, nanocomposites containing silica modified with silver nitrate reduce yeast viability with increasing content of polyurethane in the matrix. This feature correlates with the rate of release of silver ions from nanocomposites to the aqueous medium.References
1. Gerashchenko I.I. Membranotropic properties of nano-sized silica.Surface. 2009. 1(16): 288.[in Russian].
2. Siora I.V. Effect of hydrophobization and modification of nanosilica by proteins and saccharides on biocompatibility of the composites on their basis.Ph.D (Chem.) Thesis. (Kyiv, 2014). [in Ukrainian].
3. Gun’koV.M., GalaganN.P., GrytsenkoI.V., ZarkoV.I., OranskaO.I., Osaulenko V.L., BogatyrevV.M., TurovV.V. Interactionofunmodifiedand partially silylated nanosilica with red blood cells. Cent. Eur. J. Chem. 2007. 5(4): 951. https://doi.org/10.2478/s11532-007-0045-5
4. Technological process of obtaining of biogenic stimulators. (Frunze: Kyrgyz State Med. Institute, 1983). [in Russian].
5. Krupska T.V., Volod’kina A.V., Gerashchenko I.I., Gladkikh Yu.V. Study of biostimulatory effect of placenta preparations by yeast growth test. Med. Chemistry. 2010. 12(2): 40. [in Ukrainian].
6. Krupskaya T.V., Gun’koV.M., Barvinchenko V.N., Turov V.V. Shulga O.V. Interaction of silica particles with cellular surface and state of interfacial water in area of contact. Ukr. Chem. J. 2008. 74(2): 84. [in Russian].
7. Johnson S., Nguyen V., Coder D. Assessment of Cell Viability. In: Current Protocols in Cytometry. (NY: Core Publ., John Wiley & Sons, Inc., 1997).
8. Lurie Y.Y. Analytical chemistry of industrial wastewater. (Moscow: Khimiya, 1984). [in Russian].
9. Pyatnitski I.V., Sukhan V.V. Analytical chemistry of silver. (Moscow: Nauka, 1975). [in Russian].
10. Korenman I.M. Photometric Analysis. Methods for the determination of organic compounds. (Moscow: Khimiya, 1970). [in Russian].
11. Yusupova A.I., Kamzolova S.V., Kozyreva T.N., Morgunov I.G. Yeast biosynthesis of succinic acid from ethyl alcohol. Ovchinnikov Bulletin of biotechnology and physical and chemical biology. 2006. 2(4): 7. [in Russian].
12. Kotenko S.Ts., Khalilov E.A., Islammagomedova E.A. Biochemical features of yeast strain Saccharomyces cerevisiae in conditions of spirit fermentations. Reports of the Samara Scientific Center of the Russian Academy of Sciences. 2010. 12(1): 721. [in Russian].
13. Putintseva O.V. Immunology: Practicum. V. 2. (Voronezh: Publishing house of Voronezh State University, 2008). [in Russian].
14. Anikeyev V.V., Lukomskaya K.A. Guide to practical lessons in microbiology. (Moscow: Prosveshchenie, 1977). [in Russian].
15. Philippovich Y.B. Fundamentals of Biochemistry. (Moscow: Agar, 1999). [in Russian].
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
