Synthesis and study of the electrokinetic potential of metal nanoparticles obtained using medicinal plant extracts
DOI:
https://doi.org/10.15407/hftp17.01.119Keywords:
“green” synthesis, gold nanoparticles, silver nanoparticles, zinc oxide nanoparticles, iron oxide nanoparticles, electrokinetic potential, medicinal plant extractsAbstract
Extracts of medicinal plants contain a set of specific compounds that can ensure the formation of metal nanoparticles and at the same time provide them with useful properties for effective use in various fields of biotechnology, in particular in agricultural production. The main goal of this work was to synthesize and study the electrokinetic potential and structural properties of green synthesis products - silver, gold, zinc oxide, and iron oxide nanoparticles, depending on the composition of the plant material used and the synthesis conditions. 18 samples of metal nanoparticles were synthesized using aqueous and alcoholic extracts of medicinal plants: eucalyptus (Eucalyptus viminalis Labill), aloe (Aloe arborescens Mill and Aloe vera L.), peppermint (Mentha?piperita L.), plantain (Plantago major L.), chamomile (Matricaria chamomilla L.), nettle (dioecious Urtica dioica L.), willow-herb (Chamerion angustifolium L.), calendula (Calendula officinalis), hibiscus (Hibiscus sabdariffa L.), and an aqueous tannin solution. All samples were characterized by the content of polyphenolic compounds. The nanoparticles were studied by microelectrophoresis, UV-visible spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), and X-ray diffraction (XRD). According to the obtained values of the electrokinetic potential, the sample synthesized using an aqueous extract of willow-herb tea proved to be the most stable among the obtained ZnO nanoparticles. Among the FexOy NPs, the most stable were nanoparticles synthesized using aqueous extracts of hibiscus and plantain. The most stable Au nanoparticles were the samples synthesized using tannin and an alcoholic extract of Aloe vera L. As for silver nanoparticles, tannin, an alcoholic extract of eucalyptus, and an aqueous extract of Aloe arborescens contributed to the formation of the most stable nanoparticles. These samples of Ag, Au, FexOy, and ZnO nanoparticles are the most promising for their further practical application, in particular in agroecology and crop production to combat phytopathogens and ensure soil health.
References
1. Rasmiya Begum S.L., Jayawardana Nadeeka U. Green synthesized metal nanoparticles as an ecofriendly measure for plant growth stimulation and disease resistance. Plant Nano Biology. 2023. 3: 100028. https://doi.org/10.1016/j.plana.2023.100028
2. Tungmunnithum D., Thongboonyou A., Pholboon A., Yangsabai A. Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: An overview. Medicines. 2018. 5(3): 93. https://doi.org/10.3390/medicines5030093
3. Bordiwala R.V. Green synthesis and applications of metal nanoparticles. A review article. Results Chem. 2023. 5: 100832. https://doi.org/10.1016/j.rechem.2023.100832
4. Puja A.M., Rupa E.J., Kim Y.J., Yang D-C. Medicinal plant enriched metal nanoparticles and nanoemulsion for inflammation treatment: a narrative review on status and future perspective. Immuno. 2023. 3(2): 182. https://doi.org/10.3390/immuno3020012
5. Mittal A.K., Chisti Y.C., Banerjee U.C. Synthesis of metallic nanoparticles using plant. Biotechnol. Adv. 2013. 31(2): 346. https://doi.org/10.1016/j.biotechadv.2013.01.003
6. Sameeh M.Y. An overview of nanoparticles from medicinal plants: synthesis, characterization and bio-applications. Adv. Biosci. Biotechnol. 2023. 14(10): 439. https://doi.org/10.4236/abb.2023.1410030
7. Saxena M., Shaikh A. Green synthesis and zeta potential measurement of silver nanoparticles. IJARIIE. 2021. 7(3): 3448.
8. Krishnamoorthi R., Bharathakumar S., Malaikozhundan B., Mahalingam P.U. Mycofabrication of gold nanoparticles: Optimization, characterization, stabilization and evaluation of its antimicrobial potential on selected human pathogens. Biocatal. Agric. Biotechnol. 2021. 35: 102107. https://doi.org/10.1016/j.bcab.2021.102107
9. Kyriakides T.R., Raj A., Tseng T.H., Xiao H., Nguyen R., Mohammed F.S., Halder S., Xu M., Wu M.J., Bao S., Sheu W.C. Biocompatibility of nanomaterials and their immunological properties. Biomed. Mater. 2021. 6(4): 42005. https://doi.org/10.1088/1748-605X/abe5fa
10. Ostolska I., Wisniewska M. Application of the zeta potential measurements to explanation of colloidal Cr2O3 stability mechanism in the presence of the ionic polyamino acids. Colloid. Polym. Sci. 2014. 292: 2453. https://doi.org/10.1007/s00396-014-3276-y
11. Jabr-Milane L., van Vlerken L., Yadav S., Amiji M. Multi-functional nanocarriers to overcome tumor drug resistance. Cancer Treatment Reviews. 2008. 34(7): 592. https://doi.org/10.1016/j.ctrv.2008.04.003
12. Parlinska-Wojtan M., Kus-Liskiewicz M., Depciuch J., Sadik O. Green synthesis and antibacterial effects of aqueous colloidal solutions of silver nanoparticles using chamomile terpenoids as a combined reducing and capping agent. Bioprocess. Biosyst. Eng. 2016. 39(8): 1213. https://doi.org/10.1007/s00449-016-1599-4
13. Dybkova S., Goncharuk O., Rieznichenko L., Terpi?owski K., Borysenko L., Tamara G., Dybkova K., Szewczuk-Karpisz K. Aqueous dispersions of 'green' silver nanoparticles for eco-applications: Synthesis, structure and biosafety. J. Mol. Liq. 2024. 415: 126319. https://doi.org/10.1016/j.molliq.2024.126319
14. Ainsworth E.A., Gillespie K.M. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nat. Protoc. 2007. 2(4): 875. https://doi.org/10.1038/nprot.2007.102
15. Dukhin S.S., Deryagin B.V. Electrophoresis. (Moscow: Nauka, 1976). [in Russian].
16. Pochapski D., Santos C., Wosiak G.L., Pulcinelli S., Santilli C. Zeta potential and colloidal stability predictions for inorganic nanoparticle dispersions: effects of experimental conditions and electrokinetic models on the interpretation of results. Langmuir. 2021. 37(45): 13379. https://doi.org/10.1021/acs.langmuir.1c02056
17. Hinterwirth H., Wiedmer S.K., Moilanen M., Lehner A., Allmaier G., Waitz Th., Lindner W., L?mmerhofer M. Comparative method evaluation for size and size-distribution analysis of gold nanoparticles. J. Sep. Sci. 2013. 36(17): 2952. https://doi.org/10.1002/jssc.201300460
18. Car J., Bla?eka D., Krstulovi N. Advanced quantitative analysis of colloidal solution of metal nanoparticles produced by laser ablation in liquids. J. Quant. Spectrosc. Radiat. Transfer. 2022. 290: 108318. https://doi.org/10.1016/j.jqsrt.2022.108318
19. Medicinal plants: Encyclopedic guide. (Ed. A.M. Hrodzinsky. Kyiv: Ukrainian Encyclopedia, Olimp, 1992). [in Ukrainian].
20. Kurizaki A., Watanabe T., Devkota H.P. Chemical constituents from the flowers of Aloe arborescens. Nat. Prod. Commun. 2019. 14(5): 1934578X1984413. https://doi.org/10.1177/1934578X19844135
21. Olennikov D.N., Ibragimov T.A., Chelombit'ko V.A. Chemical composition of Aloe arborescens and its change by biostimulation. Chem. Nat. Compd. 2009. 45: 478. https://doi.org/10.1007/s10600-009-9405-z
22. Sierra-Garc?a G.D., Castro-R?os R., Gonz?lez-Horta A., Lara-Arias J., Ch?vez-Montes A. Acemannan, an extracted polysaccharide from Aloe vera: A literature review. Nat. Prod. Commun. 2014. 9(8): 1217. https://doi.org/10.1177/1934578X1400900836
23. Park J.Y., Kim J.Y., Son Y.G., Kang S.D., Lee S.W., Kim K.D., Kim J.Y. Characterization of chemical composition and antioxidant activity of Eucalyptus Globulus leaves under different extraction conditions. Appl. Sci. 2023. 13(17): 9984. https://doi.org/10.3390/app13179984
24. Qiujian Y., Zongcai F., Liping H., Jingwei H., Zhongliu Zh., Fang L. Ellagic acid (EA), a tannin was isolated from Eucalyptus citriodora leaves and its anti-inflammatory activity. Med. Chem. Res. 2021. 30: 2277. https://doi.org/10.1007/s00044-021-02806-6
25. Fedorova H.V. Synopsis of lectures on the discipline "Chemistry (organic, physical, colloidal)". (Odesa: ODEKU, 2018). [in Ukrainian].
26. Priyadarsini K.I., Khopde S.M., Kuma, S.S., Mohan H. Free radical studies of ellagic acid, a natural phenolic antioxidant. J. Agric. Food Chem. 2002. 50(7): 2200. https://doi.org/10.1021/jf011275g
27. Lopes L.C.S., Lucas M., Brito L.M., Bezerra T.T., Gomes K.N., Carvalho F.A.A., Chaves M.H., Cantanh?de W. Silver and gold nanoparticles from tannic acid: Synthesis, characterization and evaluation of antileishmanial and cytotoxic activities. Annals of the Brazilian Academy of Sciences. 2018. 90(3): 2679. https://doi.org/10.1590/0001-3765201820170598
28. Shankar S., Ahmad A., Sastry M. Geranium leaf assisted biosynthesis of silver nanoparticles. Biotechnol. Prog. 2003. 19(6): 1627. https://doi.org/10.1021/bp034070w
29. Mahmudin L., Suharyadi E., Utomo A., Abraha K. Optical properties of silver nanoparticles for surface plasmon resonance (SPR)-based biosensor applications. J. Mod. Phys. 2015. 6(8): 1071. https://doi.org/10.4236/jmp.2015.68111
30. Berezhnytska O.S., Snihur M.D., Chygyrynets O.E., Rohovtsov O.O. Green synthesis of silver nanoparticles and their spectral properties. UCJ. 2022. 88(9): 42. https://doi.org/10.33609/2708-129X.88.09.2022.41-51
31. Yallappa S., Manjanna J., Peethambar S.K., Rajeshwara A.N., Satyanarayan N.D. Green synthesis of silver nanoparticles uing Acacia farnesiana (Sweet Acacia) seed extract under microwave irradiation and their biological assessment. J. Clust. Sci. 2013. 24: 1081. https://doi.org/10.1007/s10876-013-0599-7
32. Chandran S.P., Chaudhary M., Pasricha R., Ahmad A., Sastry M. Synthesis of gold nanoparticles and silver nanoparticles using Aloe Vera plant extract. Biotechnol. Prog. 2006. 22(22): 577. https://doi.org/10.1021/bp0501423
33. Car J., Krstulovi'c N. Analytical model for determination of size-distribution of colloidal silver nanoparticles from surface plasmon resonance wavelength and dielectric functions. Nanomaterials. 2022. 12(19): 3474. https://doi.org/10.3390/nano12193474
34. Matus M., H?kkinen H. Understanding ligand-protected noble metal nanoclusters at work. Nat. Rev. Mater. 2023. 8: 372. https://doi.org/10.1038/s41578-023-00537-1
35. Tan S.C.L., He Z., Wang G., Yu Y., Yang L. Protein-templated metal nanoclusters: molecular-like hybrids for biosensing, diagnostics and pharmaceutics. Molecules. 2023. 28(14): 5531. https://doi.org/10.3390/molecules28145531
36. Neutronography of magnetic fluid systems: a monograph. (Chernobyl: Institute for Safety Problems of NPP, 2015). [in Ukrainian].
37. Herrera-Becerra R., Rius J., Zorrilla C. Tannin biosynthesis of iron oxide nanoparticles. Appl. Phys. A. 2010. 100: 453. https://doi.org/10.1007/s00339-010-5903-x
38. Annamuhhamedova O.O., Annamuhammedov A.O. Medical plants. Hand book. (Zhytomyr: Ivan Franko ZhSU, 2014). [in Ukrainian].
39. Kumar B., Smita K., Cumbal L., Debut A. Biogenic synthesis of iron oxide nanoparticles for 2-arylbenzimidazole fabrication. J. Saudi Chem. Soc. 2014. 18(4): 364. https://doi.org/10.1016/j.jscs.2014.01.003
40. Madivoli E.S., Kareru P.G., Maina E.G., Nyabola A.O., Wanakai S.I., Nyang'au J.O. Biosynthesis of iron nanoparticles using Ageratum conyzoides extracts, their antimicrobial and photocatalytic activity. SN Appl. Sci. 2019. 1: 500. https://doi.org/10.1007/s42452-019-0511-7
41. Demirezen D.A., Yilmaz S., Y?lmaz D.D., Y?ld?z Y.?. Green synthesis of iron oxide nanoparticles using Ceratonia siliqua L. aqueous extract: improvement of colloidal stability by optimizing synthesis parameters, and evaluation of antibacterial activity against Gram-positive and Gram-negative bacteria. Int. J. Mater. Res. 2022. 13(10): 849. https://doi.org/10.1515/ijmr-2022-0037
42. Pharmaceutical encyclopedia. [Electronic resource http://www.pharmencyclopedia.com.ua]. (National Pharmaceutical University: MORION, 1999-2024). [in Ukrainian].
43. Yusof H.M., Rahman N.A., Mohamad R., Zaidan U.H., Samsudin A.A. Biosynthesis of zinc oxide nanoparticles by cell biomass and supernatant of Lactobacillus plantarum TA4 and its antibacterial and biocompatibility properties. Sci. Rep. 2020. 10: 19996. https://doi.org/10.1038/s41598-020-76402-w
44. Pehlivanoglu S., Acar C.A., Donmez S. Characterization of green synthesized flaxseed zinc oxide nanoparticles and their cytotoxic, apoptotic and antimigratory activities on aggressive human cancer cells. Inorg. Nano-Metal Chem. 2021. 53(9): 1022. https://doi.org/10.1080/24701556.2021.1980034
45. Senthilkumar N., Nandhakumar E., Priya P., Soni D., Vimalane M., Potheher I.V. Synthesis of ZnO nanoparticles using leaf extract of Tectona grandis (L.) and their anti-bacterial, anti-arthritic, anti-oxidant and in vitro cytotoxicity activities. New J. Chem. 2017. 41(18): 10347. https://doi.org/10.1039/C7NJ02664A
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Copyright (c) 2026 L.S. Rieznichenko, V.I. Podolska, L.M. Yakubenko, Т.G. Gruzina, N.I. Grishchenko, O.Yu. Voitenko, S.M. Dybkova
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